DETAILED ASSESSMENT OF DONOR XENOMATERIAL BY RAMAN SPECTROSCOPY
Pavel Е. Timchenko,1, Еlena V. Timchenko,1, Еlena.V. Pisareva,1, Michae. Y. Vlasov,2, Оleg О. Frolov,1, Larisa Т. Volova,2, R.Т. Samigullin,1, A.A. Gnedova,1 1 Samara National Research University named after Academician S.P. Korolev, Samara, Russia 2 Samara State Medical University, Institute of Experimental Medicine and Biotechnology, Samara, Russia
Abstract
The search for new ways to obtain biocompatible materials from supporting connective tissues with a given set of physicochemical and mechanical properties possessing osteoinductive properties is an urgent task of modern biotechnology and tissue engineering.
Dental tissues are promising and available sources for these purposes, and can be used in dentistry, traumatology and orthopaedics, and oral and maxillofacial surgery. The mineral component obtained by demineralisation of mammalian dental tissues is used to fill defects after injuries and trauma, to regulate metabolic processes, has pronounced regenerative properties. Also the given material can be used as a component of toothpastes and various professional dental compositions for prevention of caries and destructive processes of enamel of a tooth as a mineral component of dental tissues along with hexagonal crystal structure of hydroxyapatite contains an amorphous phase. Therefore, there is a need to assess the quality of xenogeneic bone-plastic materials from animal dental tissues.
The aim of the work was to assess in detail the donor xenogeneic material obtained from molars and incisors that have undergone primary treatment and demineralisation using Raman spectroscopy.
The results obtained may indicate that enamel hydroxyapatite has a highly crystalline calcium phosphate structure, which makes incisor enamel denser and slower to dissolve when exposed to acids. Studies have shown that both incisors and molars can be used for xenomaterials.
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Samigullin Ravil
Samara National Research University named after Academician S.P. Korolev
Russia
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