Spectral evaluation of biomaterials from juvenile dentin of various degrees of demineralization using Raman spectroscopy
Elena V. Timchenko, 1, Pavel E. Timchenko, 1, Larisa T. Volova, 2, Maksim A. Zybin, 3, Mikhail Y. Vlasov, 2, Oleg O. Frolov, 1, Svetlana S. Shipko, 1
1. Samara National Research University, Samara, Russia
2. Samara State Medical University, Samara, Russia
3. Dental clinic «Diamant», Samara, Russia
Abstract
Osteoplastic materials are the basis for the formation of the patient's own tissue. These materials serve as a biocompatible matrix for building bone tissue during its regeneration and should be completely replaced by the patient's own tissue. Dentin is biocompatible and bone tissue is formed after its transplantation. Experiments on optical evaluation using Raman spectroscopy of biomaterials from juvenile dentin demineralized with hydrochloric acid solutions with varying degrees of normality have been carried out. The objects of research were dentin obtained from healthy juvenile teeth pre-mechanically treated. Each sample was cut into 2 equal parts and further divided into two groups, according to the stages of their processing: group 1 – biomaterials from juvenile dentin demineralized in hydrochloric acid of 1.2N degree of normality; group 2 – biomaterials from juvenile dentin demineralized in hydrochloric acid of 1.8N degree of normality. Demineralization was carried out in a hydrochloric acid solution using the "LIOPLAST" technology. It is shown that in the process of demineralization of juvenile teeth, the degree of normality of the hydrochloric acid solution causes minor structural changes. With demineralization of 1.2N with hydrochloric acid solution, a greater number of organic components are preserved compared to demineralization with a hydrochloric acid solution of a normality degree of 1.8 N.
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Svetlana Shipko
Samara National Research University
Russia
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