Freshwater flatworms as a promising biological model for biophotonics
Kharlampy P. Tiras, 1,2, Kirill N. Novikov, 3, Vladimir L. Voeikov, 3, Vladimir V. Apyari, 4, Olga V. Burlakova, 3, Alexander N. Velikanov, 3
1 Institute of Theoretical and Experimental Biophysics, RAS, Pushchino
2 Pushchino State Institute of Natural Science
3 Lomonosov Moscow State University, Faculty of Biology
4 Lomonosov Moscow State University, Faculty of Chemistry
Abstract
Planarians are one of the classical models of morphogenesis: regeneration, asexual reproduction and phagocytosis. For the first time, ultra-weak photon emission (UPE) of planarians was discovered in 2001, in vivo during planarian phagocytosis after feeding (Tiras at. al., 2001). Later, in 2015, it was shown for the first time that planarian UPE is also recorded during regeneration, using the Biotox-7A chemiluminometer, and the intensity peak was directly related to the peak of proliferation of planarian stem cells - neoblasts (Tiras at al., 2015, 2018). At the same time, it was shown that when planarians are cut into several parts, the level of UPE increases, since repeated operations lead to an increase the population of proliferating cells.
Using this model, it was also shown that the spectral characteristics of planarians during phagocytosis and regeneration can also be recorded using the GretagMacbeth Eye-One Pro portable digital microspectrophotometer (Apyari at al., 2021). In particular, it has been shown that the level of hemoglobin in the body of planarians rises after feeding, and then gradually falls as the food is digested. These data allow us to consider a planarian as a promising biological model for studying various aspects of biophotonics in vivo.
Speaker
Kharlampy Panteleyevich Tiras
Institute of Theoretical and Experimental Biophysics, Pushchino State Institute of Natural Science
Russia
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