Diffuse reflectance spectroscopy for biomedical applications

Abstract

Diffuse reflectance spectroscopy can be used to analyze the optical properties of biological tissues [1], as well as to obtain information about the state of biological tissues directly during a surgical operation [2].
In this work, using the developed experimental setup, diffuse reflectance spectra of liquid and solid substances are recorded. The basis of the experimental setup is an infrared quantum-cascade laser in the range of 5.3–12.8 μm with a peak power of up to 150 mW.
We use diffuse reflectance spectra to identify different substances. For the measured diffuse reflectance spectra, the transmission spectra are modeled. The transmission spectra were modeled in two ways: using the Kramers – Kronig relations and using the Lorentz dispersion model. The efficiency of modeling diffuse reflectance spectra and transmission spectra by both methods is compared.
Diffuse reflectance spectroscopy allows noninvasively in-situ and for a time not exceeding a few seconds to obtain information about the chemical composition of the tissue under study. The listed capabilities of the method allow it to be used for a number of biomedical applications
[1] Douven L. F. A., Lucassen G. W. Retrieval of optical properties of skin from measurement and modeling the diffuse reflectance //Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical. – SPIE, 2000. – Т. 3914. – С. 312-323.
[2] Akter S. et al. Medical applications of reflectance spectroscopy in the diffusive and sub-diffusive regimes //Journal of Near Infrared Spectroscopy. – 2018. – Т. 26. – №. 6. – С. 337-350.

Speaker

Dmitriy Anfimov
Bauman Moscow State Technical University
Russian Federation

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