Carbon dots genotoxicity evidenced by coherent anti-Stokes Raman spectroscopy
Kirill A. Laptinsky1, Sergey A. Burikov1,2, Tatiana A. Dolenko1,2 1 Skobeltsyn Institute of Nuclear Physics, Lomonosov Moscow State University, Russia 2 Faculty of Physics, Lomonosov Moscow State University, Russia
Abstract
The success of the last decade in the development of new nanomaterials and their application in biomedicine is confirmed by the active growth in the number of applications for registration of new drug agents using nanoparticles in the U.S. Food and Drug Administration (FDA). According to the 2020 report [1], the agency has received more than six hundred applications since 1970, and more than half of them in the last 10 years. There is no doubt, that for the successful use of nanoparticles and nanoagents based on them in everyday medical practice, a comprehensive study of the mechanisms of interaction of nanoparticles with the environment is necessary. Due to their properties, carbon nanoparticles such as carbon dots (CDs) have broad prospects for use in various fields, including biomedicine. This is primarily due to their intense and stable fluorescence and low toxicity. However, in the literature there are conflicting data on the effect of CDs on the viability of biomacromolecules.
In this work, the effect of CDs on the calf thymus DNA chains was studied. CDs were synthesized by the hydrothermal method. Citric acid and an aqueous solution of ethylenediamine were used as precursors. Using coherent anti-Stokes Raman scattering (CARS) spectroscopy, it was found that at high concentrations, CDs have a significant effect on the structure of DNA, which manifests itself in a shift of the band with a maximum at 1093 cm-1, corresponding to vibrations of the phosphate backbone, to the region of lower wavenumbers. This shift is due to the occurrence of oxidative stress on the DNA strand. The toxic effect of CDs on DNA strands and the fundamental possibility of testing the genotoxicity of intensely fluorescent nanoparticles by CARS spectroscopy have been demonstrated.
This study was supported by the Russian Science Foundation (project No. 20-72-00144).
1.
https://www.fda.gov/media/140395/download
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Kirill Laptinskiy
Skobeltsyn Institute of Nuclear Physics
Russia
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