Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers
Gennady A. Meerovich,1,2 Ekaterina V. Akhlyustina,2, Dmitry S. Denisov,3 Ekaterina G. Kholina, 3 Elena A. Makarova, 4 Evgeny A. Lukyanets, 4 Victor B. Loschenov, 1,2 Marina G. Strakhovskaya, 3,5 1 Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia 2 National Research Nuclear University “MEPHI”, Moscow, Russia 3 Lomonosov Moscow State University,Moscow, Russia 4 Organic Intermediates and Dyes Institut, Moscow, Russia 5 Federal Scientific Clinical Center for Specialized Types of Medical Assistance and Medical Technologies for the Federal Medical and Biological Agency, Moscow, Russia
Abstract
The aim of this work is the study of antibacterial photodynamic inactivation (APDI) of Escherichia coli (on the example of a model Escherichia coli K12 TG1), the influence of the charge of photosensitizer (PS) molecules on the efficiency of binding with these Gram-negative bacteria. The results obtained confirm that PSs based on polycationic phthalocyanines and synthetic bacteriochlorins provide effective photodynamic inactivation of Escherichia coli, much higher than mono-cationic methylene blue and electro-neutral bacteriochlorin derivative. The efficiency of APDI, assessed by the intensity of bioluminescence of model Escherichia coli K12 TG1 bacteria, correlates with the charge of PS molecules and the efficiency of electrostatic binding of PS to bacterial cells.
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Ekaterina Akhlyustina
National Research Nuclear University “MEPHI”
Russia
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