Studies of the fluorescence kinetics of indocyanine green in nanoscale structures depending on the bioenvironment in vivo
Farrakhova D.S.1, Maklygina Yu.S.1, Romanishkin I.D.1, Ryabova A.V.1,2, Yakovlev D.V.1,3, Bezdetnaya L.4,5, Loschenov V.B.1,3
1. Prokhorov General Physics Institute of the Russian Academy of Science, 119991 Moscow, Russia;
2. National Research Nuclear University “MEPhI, 115409 Moscow, Russia;
3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, 117997 Moscow, Russia;
4. Centre de Recherche en Automatique de Nancy, CNRS, Université de Lorraine, 54519 Vandoeuvre-lès-Nancy, France;
5. Institut de Cancérologie de Lorraine, 54519 Vandoeuvre-lès-Nancy, France;
Abstract
Methods of optical spectroscopy, especially with time resolution, for the analysis of the state and tissue functions, in particular soft tissues of the brain, have significant advantages over other methods used in neurosurgery and neuro-oncology. The most important advantages is the high speed of information processing. No less important is the wide range of physiological and morphological parameters available for analysis by optical spectroscopy methods. Molecules and nanoparticles with fluorescent properties, photo-cytotoxic effect and selective accumulation by various cell structures and tumor tissues in comparison with surrounding tissues could significantly increase the diagnostic information content of the approach and enhance the therapeutic effect due to the selective cytotoxic effect on cancer cells. Indocyanine green is the most common fluorescence dye approved for clinical use, used in surgery for fluorescent imaging in the infrared range: mapping of sentinel lymph nodes; intraoperative visualization of head and neck lesions. An organic dye based on H-aggregates of indocyanine green opens up truly unique opportunities in the development of new technologies for sensing and imaging based on fluorescence. In the mouse tumor model, two components of the ICG fluorescence lifetime in molecular and nanoforms were observed. The fluorescence lifetimes for the ICG molecular form were 0.90±0.02 ns and 0.48±0.02 ns for the monomeric component of the molecular solution and 1.09±0.10 ns and 0.26±0.05 ns for the H-type dimers of the molecular solution, and two fluorescence lifetime components for the ICG nanoform of 1.25±0.09 ns and 0.28±0.02 ns corresponding to H-type aggregates. A correspondence was noted between the temporal characteristics of the H-aggregates fluorescence of the indocyanine green molecular solution and the indocyanine green colloidal solution accumulated in the mouse tumor model.
ACKNOWLEDGMENT
The reported study was funded by RFBR according to the research project № 21-52-15025.
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Dina Farrakhova
Prokhorov General Physics Institute of the Russian Academy of Science
Russia
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