Blood toxicity and vascular distribution of magnetic mineral submicron vehicles in vivo

Abstract

Abstract
This research is the first step in the treatment of breast cancer in vivo. Breast cancer is the most common tumor among women. Saratov State University scientists intend to conduct therapy using submicron CaCO3 particles loaded with magnetite nanoparticles.
In the beginning we were used Doppler Optical Coherence Tomography (DOCT) and Speckle-contrast imaging methods. Using these methods, we proved that the selected dosage of the particle suspension does not clog the vessels that feed the mammary gland. The study was carried out within one hour. The animal was under general anesthesia. The particle suspension was injected through a polyethylene catheter inserted into the femoral artery. The DOCT method was used for the detection of the outlet arteria epigastrica caudalis. The result showed a slight change in the diameter of the vessel. The Speckle-contrast imaging method was chosen for the analysis of smaller vessels lying near the mammary gland of mice. The experiment showed that there is a decrease in the blood flow velocity, but there is no complete thrombosis of the vessels.
After that, we could start to do an empirical in vivo study with mice. The study was carried out within 28 days. There were three groups of mice: 1) intact animals; 2) after intra-arterial injection suspension of submicron CaCO3 particles; 3) mice after intra-arterial injection with the same kind of particles containing magnetite nanoparticles. The toxicity of the particles assesses by the indicators of blood corpuscles using the automatic hematology analyzer using URIT-3020. Based on the analysis of blood cells, we concluded that the suspension of submicron particles is not toxic to the animal body in the selected dosage.


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Shushunova Natalia A.
Saratov State Univercity
Россия

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