The photonic microsphere based sensor of specific antibodies
PAWEŁ WITYK,1 PAULINA LISTEWNIK,2 AND MAŁGORZATA SZCZERSKA2
1 Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland
2 Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, Narutowicza Street 11/12, 80-233 Gdańsk, Poland
Abstract
The photonic sensor of specific antibodies
Abstract: The photonic sensor has the potential to facilitate the detection of specific antibodies. We present photonic microsphere based sensor which was used to detect specific antibodies.
Fiber-optic microspheres are microscopic light spheres widely used in research and various kinds of science. Due to the development of optical fiber technology in recent years, they are increasingly often used in the construction of fiber-optic sensors. They are mainly used as a microresonator and whispering gallery mode sensors. However, research published in the last few years showed the new possibility of using such devices.
We presente the results of optical measurements using our new sensor that detects specific antibodies. The method of operation of the sensor is based on the measurement of radiation absorption in the range of ~ 1300 nm. In order for the sensor to selectively detect specific antibodies, the bioactive film was coated on the fiber head.
Our preliminary studies allowed us to observe concentrations of specific antibodies as low as 1 µg/mL. At the same time, it is worth emphasizing that the methodology used to prepare the sensor, in which a sphere is used, allows the sensor to be verified in a continuous mode and eliminates the need to use mirrors or other elements ensuring light reflection allowing for strict temperature control and low sample volume. As a result, the sensor is very cheap to use and extremely easy to manufacture, including the use of generally available and used in optoelectronics optical fibers.
Financial support of these studies from Gdańsk University of Technology by the 11/2020/IDUB/I.3/CC grant is gratefully acknowledged. The author acknowledges the financial support of the DS Programs of the Faculty of Electronics, Telecommunications and Informatics of the Gdańsk University of Technology.
Speaker
Malgorzata Szczerska
Gdańsk University of Technology
Poland
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