Study of optical properties of the plasma in the presence of solutions of glycerol and propylene glycol by Raman spectroscopy
Arina A. Sokova1, Ekaterina N. Lazareva 1,2, Natalia A. Shushunova 1, Alla B. Bucharskaya 1,2,3, Valery V. Tuchin 1,2,4 1 Saratov State University, Saratov, Russia 2 Tomsk State University, Tomsk, Russia 3 Saratov State Medical University, Saratov, Russia 4 Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
The use of non-invasive methods of diagnosis and treatment in medicine to this day does not lose its relevance. Raman spectroscopy is one of the methods for analyzing the molecular composition of a substance, in particular, blood and its constituents (plasma). Blood serum contains almost 90% water, about 6.6-8.5% proteins and other organic and mineral compounds, which are intermediate or end products of metabolism carried by the blood. Various blood components have a significant impact on the Raman spectra [1, 2].
In this study, the minimum limiting blood plasma concentrations were identified when glycerin and propylene glycol solutions were added. The study was carried out in the presence of solutions of glycerin and propylene glycol at different concentrations. Plasma and glycerol solutions had a concentration of 0%, 1%, 2%, 3%, 5%, 7%, 10%, 20%. Plasma and propylene glycol solutions: 0%, 1%, 2%, 5%, 10%, 20%. In addition, preliminary research was carried out separately for solutions of glycerin in percentages: 0%, 1%, 2%, 5%, 10%, 20%, 40%, 60%, 80%, 100%; and for a propylene glycol solution in the same percentage. The second component in these solutions is physiological solution (an aqueous solution of sodium chloride (NaCl) with a mass fraction of ≈ 0.9%)
Based on the obtained dependences of the Raman spectrum intensity on the concentration of solutions, the minimum limiting concentrations were revealed. For glycerin solution - 5%; for blood plasma with the presence of glycerin - 3%; for propylene glycol solution - 2%; for blood plasma with the presence of propylene glycol - 10%.
The reported study was funded by a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
[1] V. V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnostics, 3rd ed., SPIE Press, Bellingham, Washington (2015).
[2] I. A. Bratchenko, D. N. Artemev, Yu. A. Khristoforova etc., Raman Spectroscopy Techniques for Skin Cancer Detection and DiagnosisCham: Springer Nature, (2020).
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Arina A. Sokova
Saratov State University
Russia
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