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Research of the transport of polarised irradiation in rat skin ex vivo during dehydration

Maria A. Grishaeva, 1 Sergey M. Zaytsev, 1 Elina A. Genina, 1,2 1 Saratov State University, Saratov, Russia 2 Tomsk State University, Tomsk, Russia


Dehydration is one of the main mechanisms of optical clearing. As a result of dehydration, there is a decrease in the thickness of the tissue sample, internal matching of refractive indices, and an enhancement of the packing and ordering of fibers. The scattering of polarized light by randomly inhomogeneous media, which are the majority of biological tissues, leads to its partial or complete depolarization. Optical clearing can significantly increase the depth of polarization imaging. The tissue clearing method is convenient, simple, and inexpensive for diagnostic purposes.
The effect of skin dehydration on the change in the degree of polarization of the probing radiation has not been studied enough. In this work, both the propagation of polarized radiation in a skin sample during its dehydration and the intensity of this radiation were investigated using a polarizing microscope.
During the experiment, the rat skin sample ex vivo was sufficiently cleared due to dehydration. In the dermis, the fibers were ordered, and the sample itself significantly decreased its thickness.
As a result of this work, the temporal dependences of the intensity of polarized radiation during dehydration of the rat skin were obtained.


Maria Grishaeva
Saratov State University


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