Application of a multimodal method for the study of human skin neoplasms with optical clearing in vivo to increase the effectiveness of PDT.
I.A. Serebryakova 1.2, Yu.I. Surkov 1,2, E.A. Genina 1,2, E.N. Lazareva 1.2, Y.K. Kuzinova 3, O. M.Konopatskova 3, A.N. Bashkatov 1.2, V.V. Tuchin 1,2,4
1 Saratov National Research State University named after N.G. Chernyshevsky
2 National Research Tomsk State University
3 Saratov State Medical University named after V.I.
Razumovsky Ministry of Health of Russia
4 Institute for Problems of Precision Mechanics and Control, RAS
Abstract
Despite the development of medicine, oncological diseases are still one of the most dangerous at the present time. For the diagnosis and therapy of neoplasms, more and more often, preference is given to non-invasive, including optical methods such as optical coherence tomography (OCT), confocal microscopy, fluorescence spectroscopy, photodynamic and laser exposure, the effectiveness of which depends on the intensity and depth of penetration of optical radiation. Optical clarifying agents (OСA) are used to change the optical properties of biological tissues. Photodynamic therapy (PDT) of malignant skin neoplasms is a promising and functionally sparing method of treatment. The use of optical brightening (OС) of the skin can promote increasing the efficiency of PDT of the skin by clarifying the localization of the tumor and increasing the depth of penetration of radiation into the tissue. The work was attended by volunteers aged 49 to 78 years, who gave an informed consent to conduct research. Areas with neoplasms (basal cell carcinoma and benign neoplasms) and nearby healthy skin areas for comparison. During the study, examination and ultrasound examination (US) were carried out using the DUB SkinScanner apparatus (tpm taberna pro medicum GmbH, Germany) with two probes operating at central frequencies of 33 and 75 MHz, the scanning depth was 5 and 3.2 mm and the longitudinal resolution was 48 and 21 μm, respectively, B-scans were obtained at using spectral OCT GAN930V2-BU (Thorlabs, USA) operating at a central wavelength of 930 nm with an axial and lateral resolution of 6 and 7.32 μm, respectively, and a scanning depth of 2 mm, and diffuse reflection coefficients were measured using USB4000-UV-VIS and NIRQUEST spectrometers (Ocean Optics, USA) and QR400-7-VIS-NIR fiber-optic probes (Ocean Optics, USA) in the ranges of 400 - 2150 nm of the studied skin areas. Raman spectra measured in the same areas using a QE65000 spectrometer (Ocean Optics, USA) equipped with a 785 nm diode laser (Ocean Optics, USA) and a probe (f = 7.5 mm) were used as a criterion for differentiating skin neoplasms. A 40% aqueous solution of glycerin was used as the OСA, which was applied to the study area for 10 minutes. The OС result was recorded using OCT before and after the application of the OСA. The obtained tomograms were used to determine the increase in the depth of light penetration into the skin under the action of OPA. The described multimodal approach makes it possible to assess the general condition of the skin, outline the boundaries of the neoplasm and estimate its size, as well as determine its size internal structure. The presented results can be useful for the optical diagnosis of skin diseases and for the refinement of PDT and laser parameters therapy for OС.
Speaker
Serebryakova Isabella Anatolevna
SSU
Russia
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