Biophotonics study of cells interaction in blood under normal and pathological conditions

Abstract

Red blood cells (RBC) microrheologic properties strongly influence the microcirculation of blood and human health in general. These properties essentially depend on RBC interactions. In this work, we characterize the RBC interaction parameters and resulting aggregation, deformability and capillary flow properties using in vitro and in vivo optical measurements. We use such biophotonics techniques as RBC aggregometry method based on diffuse light scattering from whole blood samples in microfluidic flow chambers, laser diffractometry technique from dilute suspensions of RBC in controlled shear flow, laser trapping and manipulation of single RBC (all in vitro), and digital capillaroscopy of blood flows in nail bed capillaries in vivo. The results of large series of measurements based on a group of healthy volunteers and several groups of patients suffering such socially important diseases as arterial hypertension and type 2 diabetes mellitus show significant impairment of microrheologic properties, in particular, enhancement of RBC aggregation both in large populations of cells and in cell doublets. A statistically significant correlation of these changes with alterations of blood capillary flow parameters was also found.
Biophotonics characterization of red blood cells microrheology and blood microcirculation performed using in-vitro and in-vivo measurements enables one to determine significant alterations in important features of human organisms that may need urgent clinically corrections.
The study was supported by the Russian Foundation for Basic Research (grant No19-52-51015) and Scientific School “Photonic and Quantum Technologies. Digital Medicine” of Lomonosov Moscow State University.

Speaker

Alexander Priezzhev
Lomonosov Moscow State University
Россия

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