Towards to the correction of erythrocyte aggregation by using dextran proagregant macromolecules
A.A. Romanova, P.B. Ermolinskiy, A.N. Semenov, A.E. Lugovtsov and A.V. Priezzhev
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia
Abstract
Red blood cell (RBC) aggregability refers to the cells' ability to form multicellular aggregates in the presence of different plasma proteins or macromolecules. Opposing forces determine the extent of aggregation by the repulsive force between the negatively charged cells, the cell-cell adhesion induced by the macromolecules, and the disaggregating flow-induced shear stress. Kinetic and dynamic features of the cells interaction can characterize the RBC aggregation process [1].
The present study is intended to examine the RBC aggregation in dextran solutions in blood plasma samples using the diffuse light scattering technique as well as on the single cell level in a highly diluted suspensions using the optical tweezers [2].
Experiments were carried out with the addition of dextran of 500 and 40 kDa molecular weight into the blood at the concentration of 50 mg/ml.
Basing on the obtained results, we can conclude that dextran with the molecular weight of 40 kDa is an inhibitor of aggregation, while dextran with the molecular weight of 500 kDa is an RBC aggregation inducer. Optical methods provided handy tools to study the kinetic and dynamic peculiarities of RBC aggregation and disaggregation, which can be extremely useful for controlling and correction the blood microcirculation especially in cases of hemorheological disorders.
The study was supported by the Russian Foundation for Basic Research grant №19-52-51015 and Scientific School “Photonic and Quantum Technologies. Digital Medicine” of Lomonosov Moscow State University.
[1] A. Semenov, A. Lugovtsov, S. Shin, G. Barshtein, and A. Priezzhev "Red blood cells interaction mediated by dextran macromolecules: in vitro study using diffuse light scattering technique and optical tweezers", Proc. SPIE 11065, Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, 110651X (2019)
https://doi.org/10.1117/12.2523744
[2] A. Lugovtsov, Yu. Gurfinkel, P. Ermolinskiy, A. Maslyanitsina, L. Dyachuk, and A. Priezzhev, "Optical assessment of alterations of microrheologic and microcirculation parameters in cardiovascular diseases," Biomed. Opt. Express 10, 3974-3986 (2019)
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Anastasia Romanova
Faculty of Physics, Lomonosov Moscow State University
Russia
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