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Comparison of the capabilities of spectroscopic and video analysis of fluorescence for the diagnosis of cholangiocellular cancer

Dmitry V. Yakovlev,1,2 Dina S. Farrakhova,1 Artem A. Shiryaev,3 Tatiana A. Savelieva,1 Igor V. Reshetov,3 Victor B. Loschenov,1,4 1 Prokhorov General Physics Institute of the Russian Academy of Science, Moscow, Russia 2 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russia 3 University Clinical Hospital no. 1, Oncology Center, I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow, Russia 4 National Research Nuclear University “MEPhI, Moscow, Russia;

Abstract

Cholangiocellular carcinoma (CCC) is a rare oncological disease of the bile ducts, which in the early stages is characterized by slow growth; invasion of regional lymph nodes and spread to the surrounding liver parenchyma. One of the main reasons for the unfavorable outcomes of CCC is the late diagnosis of the disease, as well as the low efficiency of modern diagnostic methods. At present, modern physical methods of theranostics - fluorescence diagnostics (FD) and photodynamic therapy (PDT) - have proven to be the most effective in radical and palliative treatment of oncological neoplasms of various localization and character. In the laboratory of laser biospectroscopy of the GPI RAS, together with I.M. Sechenov First Moscow State Medical University, two methods and devices for their implementation have been developed - for spectroscopic diagnostics LESA-01-BIOSPEC (OOO BIOSPEC) and for video-fluorescent diagnostics UFF-630 / 675-01-BIOSPEC (OOO BIOSPEC). Spectroscopic analysis is the most sensitive, but it has a number of significant drawbacks, such as the lack of the possibility of visual control of the surgical field, which is an essential parameter within the framework of this localization. Within the framework of this study, a comparative analysis was carried out using the methods of mathematical statistics for these diagnostic methods. A correlation was noted between the data on the concentration of the fluorophore, determined by two different methods, before the PDT procedure and after PDT. It is shown that the video-fluorescent method for diagnostics of CCC is comparable in sensitivity to the spectroscopic method of investigation.

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Dmitry Yakovlev
1. Prokhorov General Physics Institute of the Russian Academy of Science, 119991 Moscow, Russia
Russia

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