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Immunofluorescence microscopy reveals expression and intracellular localization of transcription factor E2F1 in the peripheral neurons of rats and crayfish Astacus Leptodactylus after axotomy

Valentina A. Dzreyan, Stanislav V. Rodkin, Maria A. Pitinova, Moez Eid
Laboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, prospect Stachki 194/1, Rostov‐on‐Don, Russia

Abstract

In this work, by immunofluorescence microscopy showed for the first time intracellular localization of E2F1 in various model objects: in axotomized mechanoreceptor neurons (MRN) and ventral nerve cord ganglia (VNC) of crayfish, as well as in the dorsal root ganglia (DRG) of the rat spinal cord in the early stages after axotomy. It is determined in which cells, neurons, or glia cells, this protein is expressed. This work showed the rabbit antibodies against mammalian E2F1 used in this work perfectly recognized the corresponding epitopes in the homologous crayfish protein.
Axotomy causes overexpression of E2F1 as early as 4 hours and even 1 hour after axotomy of MRN and ganglia of crayfish VNC, as well as rat DRG. It was interesting to note that E2F1 was expressed exclusively in neurons, but not in the glial cells of the rat DRG ganglia and the crayfish MRN.
Thus, we studied the expression of E2F1 in three models of neurotrauma in vertebrates and invertebrates. It has been demonstrated that E2F1 is present not only in the nervous system of invertebrates, but also in crustaceans, confirming the conservative nature of this protein. Our novel date by immunofluorescence microscopy indicate that E2F1 can be potential biomarker in the in the early stages after neurotrauma, suggesting that inhibitors of E2F1 can be considered for therapeutic approaches in this period.
Supported by a grant from the Ministry of Education and Science of the Russian Federation No. 0852-2020-0028 and a stipend from the President of the Russian Federation for young researchers.


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Dzreyan Valentina Aleksandrovna
Laboratory of Molecular Neurobiology, Southern Federal University, prospect Stachki 194/1, Rostov‐on‐Don, Russia
Russia

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