CHEMOMETRICS-ASSISTED SPECTROPHOTOMETRIC DETERMINATION OF SYNTHETIC FOOD COLORANTS IN "STREPSILS" LOZENGES

Abstract

Preparations with an antiseptic effect for the treatment of pain in the mouth and throat are especially relevant in the autumn-winter period. The composition of these drugs, in addition to the active substances with antiseptic action, also include auxiliary substances, for example, synthetic food colorants (SFC). The need to control the amount of SFC consumed is an important task, since exceeding the permissible daily intake can lead to allergic reactions, hyperactivity in children.
The aim of this work is to use the spectrophotometry method in combination with chemometric algorithms to determine E110 and E124 colorants in the Strepsils lozenges.
Two chemometric methods applied were partial least squares (PLS) and principal component regression (PCR). These approaches were used to quantify the colorants in model colorants solutions and Strepsils lozenges aqueous solutions based on information included in the visual absorption spectra. The calibration PLS-1, PLS-2 and PCR models were evaluated by internal validation (prediction of compounds in its own designed training set of calibration) and by external validation over pharmaceutical preparation. The accuracy of the methods was evaluated through the root mean square error of prediction (RMSEP). The results of PLS-1 and PCR methods were compared and the best models for the system were obtained with PCR.
Ultimately, a method was developed for the chemometric-assisted spectrophotometric determination of E110 in the presence of E124 in the "Strepsils" tablets, which is characterized by simplicity and rapidity.

The work was supported by Russian Foundation for Basic Research (project number 20-33-90097).

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Dmitrii Silaev
Saratov State University
Russia

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