Effect of duration of circadian rhythm disturbance by light exposure on liver morphology of laboratory rats

Abstract

In the modern world, people are increasingly in conditions of irregular working hours and regularly forced prolonged exposure to artificial light during the dark hours of the day. Changing light regime is a powerful stressogenic factor inducing the development of both functional and structural disorders, significantly increasing the risk of various pathologies. Objective of the investigation: studying the effect of long-term constant light exposure on severity and reversibility of morphological changes in the liver. Modelling of long-term light exposure was carried out by round-the-clock exposure to artificial light on 60 male white mongrel rats. The experiment was divided into two series: the first was devoted to study the effect of light exposure duration on liver morphology, the second - aimed to study the reversibility of the changes detected. It was found that under prolonged light exposure in the liver damage of parenchyma (dystrophy and necrosis of hepatocytes), moderate blood flow with intravascular hemolysis of erythrocytes and accumulation of hemosiderin grains in Kupffer cells develops. When studying reversibility on the 14th day after termination of light exposure in both experimental groups restoration of the organ structure was observed: reduction of stromal edema and full-thickness of vessels, decrease of dystrophy and necrosis severity of hepatocytes. It was found out that expression of morphological changes developing in the liver depended on the duration of light stimulation. After providing natural light in the laboratory, a gradual recovery of the morphological structure of the liver was observed, which testifies to the reversibility of the revealed changes.

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Speaker

Pakhomy S.S.
Saratov State Medical University named after V.I. Razumovsky
Russia

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