Study of changes of the optical properties of rat blood during inhalation of e-cigarette liquid aerosol by Raman spectroscopy and microscopic
Ekaterina N. Lazareva 1,2, Polina A. Dyachenko1,2, Alexander E. Polozhenkov3, Lyudmila I. Kozakova3, Artyom M. Mylnikov3, Alla B. Bucharskaya 1,2,3, Valery V. Tuchin 1,2,4 1 Saratov State University, Saratov, Russia, 2 Tomsk State University, Tomsk, Russia , 3 Saratov State Medical University, Saratov, Russia , 4 Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
The actual problem of our time is the replacement of tobacco smoking with the use of devices with smoking mixtures. However, scientists have proven that their use also has a negative effect on the human body, in particular on the mucous membranes of the larynx and nasal cavity, the central nervous system, blood, and causes irreversible destructive processes, such as the development of cancer and other diseases [1, 2]. A deep and detailed study of the effects of smoking mixtures on the body of animals will make it possible to better understand what processes are involved in this. It should also be noted that e-liquids are the main antireflection agents in optics [3, 4]. Their effect on tissues makes it possible to increase the depth of sensing of laser radiation and to increase the efficiency of the used optical methods [5].
This pilot study shows the results of a study of the optical properties of whole blood of rats when exposed to smoke from a smoking mixture based on propylene glycol and glycerin by the methods of Raman scattering (RS) and microscopy. Whole blood was obtained from the tail vein of laboratory animals before and after inhalation. An increase in the RS signal and a decrease in the size of red blood cells indicate the accumulation of components of e-liquid in the blood and is a reason for continuing the study.
The reported study was funded by a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
[1] Didkowska, J., Wojciechowska, U., Manczuk, M., Lobaszewski, J., 2016. Lung cancer epidemiology: contemporary and future challenges worldwide. Ann. Transl. Med. 4, 150.
[2] Restrepo, M.I., Chalmers, J.D., Song, Y., Mallow, C., Hewlett, J., Maldonado, F., Yarmus, L., 2017. Year in review 2016: respiratory infections, acute respiratory distress syndrome, pleural diseases, lung cancer and interventional pulmonology. Respirology 22, 602–611
[3] Zhu, D., Larin, K.V., Luo, Q. & Tuchin, V.V. (2013) Recent progress in tissue optical clearing. Laser & Photonics Reviews, 7(5), 732–757.; Tuchin, V.V. (2015a) Tissue optics and photonics: Light-tissue interaction. Journal of Biomedical Photonics & Engineering, 1, 98–134.
[4] Susaki EA, Ueda HR. Whole-body and Whole-Organ Clearing and Imaging Techniques with Single-Cell Resolution: Toward Organism-Level Systems Biology in Mammals. Cell Chem Biol. 2016 Jan 21;23(1):137-157.
[5] Tian T, Yang Z, Li X, et al. Tissue clearing technique: Recent progress and biomedical applications. J Anat. 2021;238:489–507.
File with abstract
Speaker
Ekaterina N. Lazareva
1 Saratov State University, Saratov, Russia 2Tomsk State University, Tomsk, Russia
Russia
Discussion
Ask question
