Spectral and time-resolved photoluminescence of human platelets doped with platinum nanoparticles
Karina I. Matveeva,1 Andrey Yu. Zyubin,1 Elizaveta A. Demishkevich,1,2 Vladimir V. Rafalskiy,1 Ekaterina M. Moiseeva,1 Igor I. Kon,1 Anna A. Kundalevich,1,2 Viktoria V. Butova,1 Ilia G. Samusev,1 1 Immanuel Kant Baltic Federal University, Kaliningrad, Russia
2 Saratov State University, Saratov, Russia
Abstract
This paper describes detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs). Fluorescence, quantum yield and platelet amino acid lifetime changes in presence and without femtosecond ablated platinum NPs have been studied.
Fluorescence spectroscopy analysis of main fluorescent amino acids and their residues (tyrosine (Tyr),tryptophan (Trp) and phenylalanine (Phe)) belonging to the platelet membrane have been performed. FDTD-based mathematical modeling for the spherical platinum NPs has been done. Fluorescence resonance energy transfer (FRET)-based quenching with corresponding energy parameters has been calculated for all complexes. The prospects of platinum NPs use for quenching-based sensing have been demonstrated.
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Demishkevich Elizaveta
Saratov State University, Immanuel Kant Baltic Federal University
Russia
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