Visualisation of ICG based bimodal contrast agent on integrated photoacoustic/fluorescence tomography setup
Maksim D. Mokrousov,1 Weylan Thompson,2 Sergey A. Ermilov,2 Tatiana Abakumova,1 Marina V. Novoselova,1 Olga A. Inozemtseva,3 Timofei S. Zatsepin,1,4 Vladimir P. Zharov,5 Ekaterina I. Galanzha,5 Dmitry A. Gorin,1
1 Skolkovo Institute of Science and Technology, Moscow, Russia
2 PhotoSound Technologies, Houston, TX, USA
3 Saratov State University, Saratov, Russia
4 Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia
5 University of Arkansas for Medical Sciences, Little Rock, AR, USA
Abstract
Fields of experimental medicine, clinical practice and preclinical research have a high demand for multimodal imaging systems. Photoacoustic imaging has been combined with other modalities such as fluorescence, ultrasound, MRI, and OCT. Combination of photoacoustic and ultrasound imaging in a single system is the only commercially available bimodal photoacoustic setup now. Photoacoustic and fluorescence modalities are naturally complement each other and this makes the combination of these modalities an attractive approach. In this work we demonstrate the indocyanine green dye (ICG) embedded in the biocompatible and biodegradable polymer shell particles as a single signalling compound bimodal contrast agent and demonstrate its characteristics by visualisation on a commercial photoacoustic/fluorescence tomography system (TriTom, PhotoSound Technologies). It was found that fluorescence signal depends on the total amount of dye in the particle and photoacoustic signal of the particles depends on the amount of dye in the shell. A commercial bimodal photoacoustic/fluorescence setup was used for characterization of polymer particles containing ICG for the first time to our knowledge. We conducted additional studies for these particles, such as cell toxicity and biodistribution over time in vivo and ex vivo using fluorescent imaging. The obtained results suggest a potential for the application of biocompatible and biodegradable bimodal contrast agents as well as the integrated photoacoustic/fluorescence imaging system for preclinical and clinical studies.
Speaker
Maksim Mokrousov
Skolkovo Institute of Science and Technology
Russia
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