Increasing the spatiotemporal resolution by phase modulation in Lissajous scanning confocal microscopy
Hamed Qazvini,1
Amir Asadollahi,1
M.Sadegh Feiz,1
Ali Fakhrolmobasheri,2
Hamid Latifi,1,2
1 Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
2 Department of Physics, Shahid Beheshti University, Tehran, Iran
Abstract
One of the challenges in scanning microscopy of dynamic biological samples is to increase imaging speed without significant reduction in spatial resolution. Using Lissajous patterns is a way to make the image available in less time via gathering scattered data from the entire FOV. In sample scanning by Lissajous, the distance between the lines in the center of the FOV is greater than in the corners, and as a result, the information obtained from the center of the scanned area will be reduced. This phenomenon increases when, in order to increase the frame rate, the number of pattern lobes is reduced. In this article, the density of scanned lines in the center of the sample has increased compared to the normal Lissajous patterns with the use of phase modulation. Thus, with preserving the image details, it is possible to increase the frame rate up to 2 times compared to conventional raster scanning. By utilization of the interpolation algorithms, the image is reconstructed according to the desired temporal and spatial resolution.
Speaker
Hamed Qazvini
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
Iran
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