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Raman Spectroscopic Study on the TiO2 Nanoparticles effects on the Hemoglobin State in Single Red Blood Cell

E.V. Perevedentseva1, 2, Y.- C. Lin1, A.V. Karmenyan1, K.-T. Wu1, A. E. Lugovtsov3, A.V. Priezzhev3, C.-L. Cheng1

1 Department of Physics, National Dong Hwa University, Hualien Taiwan
2. P. N. Lebedev Physics Institute of Russian Academy of Sciences, Moscow, Russia
3 Department of Physics, Moscow State University, Moscow, Russia


Titanium dioxide is considered as a non-toxic material and widely used in number of everyday products, such as sunscreen. Titanium dioxide nanoparticles (NP) are also considered as prospective agent for photodynamic therapy and drug delivery. These applications require understanding of Titanium dioxide potential effects on blood system and its components upon administration. In the presented work we analyze the interaction of Titanium dioxide nanoparticles of different crystal phases (anatase and rutile) with individual rat Red Blood Cells (RBC) and the Titanium dioxide influence on the oxygenation state and functionality of RBC, estimated via analysis of Raman spectra of Hemoglobin (Hb) and their distribution along individual RBC. Raman spectral signals allows localization of the Titanium dioxide NP on the RBC. No penetration of the NP inside RBC was observed, however both kinds of Titanium dioxide NP adsorbed on the RBC membrane that can affect the Hb state. Mechanisms involving the NP-membrane-Hb interaction resulting in partial deoxygenation of Hb and Titanium dioxide photothermal effect on Hb under Raman excitation wavelength are suggested. Possible influence on the safety at Titanium dioxide use in advanced medical application, especially on safety and efficiency of photothermal therapy, are discussed.


Elena Perevedentseva
Physics Department, National Dong Hwa University


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