The novel photosensitizer galactose – Lutetium (Lu)-phthalocyanine for photodynamic therapy in tumor-bearing mice
Alla B. Bucharskaya,1,2,3, Gаlina N. Maslyakova,1,2, Nikita A. Navolokin,1,2, Georgy S.Terentyuk,2, Ekaterina Borisova,4, Vanya Mantareva,5, Ivan Angelov,5, Vadim Genin,2, Alexey N. Bashkatov,2,3, Elina A. Genina,2,3, Valery V. Tuchin2,3,6
1Saratov State Medical University, Saratov, Russia; 2Saratov State University, Saratov, Russia; 3Tomsk State University, Tomsk, Russia; 4Institute of Electronics, Bulgarian Academy of Sciences, Bulgaria; 5Institute of Organic Chemistry with Center on Phytochemistry, Bulgarian Academy of Sciences, Bulgaria; 6Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
For photodynamic therapy (PDT), galactose – Lutetium (Lu)-phthalocyanine, as a novel photosensitizer, was applied by intratumoural injection in 8 outbred albino male mice with inoculated hepatoma in a dose of 2 mg/kg. Then, tumors were irradiated through the skin by 670 nm-diode laser source with irradiation dose 200 J /cm2 applied for 1000 sec.
The withdrawal of the animals from the experiment and sampling of tissues for morphological study were performed before (n=4) and 72 hrs after PDT (n=4). Tissue of the tumor was taken for morphological examination and fixed in 10% formalin. Serial paraffin sections were stained with hematoxylin-eosin and with the use of immunohistochemical staining with antibodies to proliferation marker Ki-67 and apoptotic marker BAX (Abcam, UK).
3 days after PDT with galactose-Lu-phthalocyanine, pronounced necrotic changes and inflammation reaction were observed in central tumor area, necrosis fields occupied up to 80% of the area. The dystrophic changes, reduction of proliferation degree and development of apoptosis in tumor cells were observed on tumor periphery. The decreased expression of proliferation marker Ki-67 and the increased expression of apoptotic marker BAX were noted after PDT.
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Speaker
Alla Bucharskaya
Saratov State Medical University n.a.V.I.Razumovsky
Russia
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