Surface-enhanced Raman scattering substrates for DNA detection based on nanogold-capped poly(DEGDMA) microparticles
Malik H. Mahmood1, 2, 3, *, Ali Jaafar1, 2, 4, László Himics1, László Péter1, Ágnes Nagyné Szokol1, István Rigó1, Shereen Zangana5, Attila Bonyár5, Miklós Veres1
1 Institute for Solid State Physics and Optics, Wigner Research Center for Physics, Budapest, H-1525, P.O.Box 49., Hungary
2 Institute of Physics, University of Szeged, Dóm tér 9, H-6720 Szeged, Hungary
3 Physiology Department, College of Medicine, University of Misan, Al-Amarah 62001, Misan, Iraq
4 Ministry of Higher Education and Scientific Research, Baghdad 10065, Iraq
5 Department of Electronics Technology, Faculty of Electrical Engineering and Informatics, Budapest University of Technology and Economics, Budapest H-1111, Hungary
*corresponding author: malik.hadi@wigner.hu
Abstract
In this study, the surface-enhanced Raman scattering (SERS) characteristics of poly(DEGDMA) microparticles coated with gold nanoparticles (AuNPs) were studied in order to determine their sensing performance. Firstly, a comparative study of SERS-active and non-SERS-active microparticles revealed that the intensities of the rhodamine 6G peaks were significantly increased due to localized plasmon resonance caused by AuNPs linked to polymeric microspheres in the SERS-active microparticles. The enhancement factor of synthesized poly(DEGDMA)/AuNP structure was determined by using various concentrations of the target analyte for different excitation wavelengths. It has been found that from the three available excitations (532, 632 and 785 nm), the 785 nm laser produces the greatest SERS signal. Furthermore, the poly(DEGDMA)/AuNPs substrate was demonstrated to be stable for more than 60 days in SERS experiments. The SERS performance of the substrate was demonstrated with the application to a DNA sequence, which also proved the possibility to utilize the poly(DEGDMA)/AuNP substrate for biosensing applications.
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Malik H. Mahmood
Wigner Research Centre for Physics
Hungary
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