Reveling kinetics of chemical transitions in colorimetric indicators of bacterial growth based on photometric data from a portative microbiological analyzer
Alexander V. Sychev, 1 Roman N. Belenkov 1, Dmitry N. Ukolov 1, Artem V. Budaev 1, Anastasia I. Lavrova 2,3 Eugene B. Postnikov 1 1 Kursk State University, Russia 2 Saint-Petersburg State Research Institute of Phthisiopulmonology, Russia 3 Saint-Petersburg State University, Russia
Abstract
We outline the construction, results of testing, and methodology of data processing for a newly developed portative microbiological analyzer (patent application RU2021111818,24.04.2021). This Arduino-based setup allows for high-frequency temporal resolution of data recordings that reduces instant noises and provides detailed dynamical curves. It was tested with the culture of baker’s yeast Saccharomyces cerevisiae in a liquid medium with the usage of resazurin as a redox indicator changing its colour. Moreover, for such fast-growing culture, one should take into account the full chain of chemical transitions from resazurin to resarufin and further to dihydroresorufin. We argue that the respective model of such chemical cascade (Gorbunova et al., 2020) can be treated with the loglet-decomposition of the recorded data into components of two Bertalanffy’s models and demonstrate the ability to define the required kinetic rates.
Speaker
Alexandr Sychev
Kursk State University
Russia
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