ADAPTIVE OPTICAL BESSEL BEAM TWO-PHOTON FLUORESENCE MICROSCOPY FOR VOLUMETRIC IMAGING OF SYNAPES IN DEEP CORTEX
WEI CHEN1, RYAN NATAN1, YUHAN YANG1 AND NA JI1
1Department of physics, UC Berkeley, USA
Abstract
Deciphering the information processing within neuronal circuities demands a technique that is capable to record multitude neuronal inputs at synaptic resolution and the dynamic of neuron ensembles across a large brain volume. In favor of its non-diffractive propagation in space, Bessel beam has been demonstrated in two-photon microscopy as an approach to improve the volumetric imaging speed via an axially elongated focus. However, the impacts of phase turbulence, such as aberrations presented in the imaging system and biological samples, are usually overlooked and less studied for Bessel beam. In this study, we have shown that aberrated Bessel beam reduces the two-photon excitation efficiency and degrades the signal-to-noise ratio (SNR) especially for small neuronal structures in deep cortical layers.
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Wei Chen
UC Berkeley
USA
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