Protein Profile study of neonatal tear fluids
Newborn babies (neonates) begin to secrete tears in the first 24 hours of life and it produces enough aqueous to maintain clear vision and humidify the ocular surface. Neonatal tear analysis can provide information about the health condition of eyes since it contains specific molecules, proteins and their relative concentrations may alter under abnormal conditions. Neonatal tear protein content has not been studied as much as in adults tear sample due to the difficulty of collecting samples without causing reflex tearing. To explore the neonatal tear samples extensively, spectroscopic, proteomic, lipidomic, and metabolomics studies are necessary. The present objective of the study is to investigate the unstimulated neonatal tear fluid by collecting samples using non-invasive method (Schirmer strip) and analyze the protein patterns of term and pre-term babies tear fluids using high performance liquid chromatography with light emitting diode- induced fluorescence system (HPLC-LED-IF) developed in our laboratory. The studies have reported concentration of standard protein markers (lysozyme, lactoferrin and serum albumin) in neonatal tear fluid are of the order of micromolar concentration. The detection limits of these standard proteins obtained for the present system are of the order of nanomolar concentrations. The protein profiles of term and preterm tear fluid samples has been analyzed and found noticeable differences between each category.
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Sphurti S Adigal
PhD Scholar,2. Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal
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