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Photodynamic, Sonodynamic and Sonophotodynamic Therapies against Staphylococcus aureus biofilm

Fernanda Alves, 1 Natália M. Inada, 1 Sebastião Pratavieira,1 Vanderlei S. Bagnato,1 Cristina Kurachia, 1
1 São Carlos Institute of Physics - University of São Paulo

Abstract

This work evaluated Antimicrobial Photodynamic Therapy (PDT), Sonodynamic Therapy (SDT) and the association of both therapies (Sonophotodynamic Therapy, SPDT), mediated by Curcumin (Cur) against Staphylococcus aureus biofilm. Next, additional strategies to these treatments were assessed. S. aureus biofilms received PDT, SDT and SPDT, mediated by Cur (80 µM), LED light (450 nm), and 1 MHz ultrasound. The same treatments were also performed adding a strategy: Cur with sodium dodecyl sulfate (SDS), Cur with potassium iodide (KI) or a pre-treatment with ultrasound. Cell viability was determined and biofilm architecture was evaluated under confocal microscopy. SPDT was more effective than PDT and SDT. SDS achieved the greatest viability reductions, followed by KI and ultrasound pre-treatment. Confocal images revealed biofilm disruption and reduced number of cells in all treatments. However, SPDT exhibited a pronounced effect and it was greater using SDS. In conclusion, SPDT was more effective and additional strategies potentiated its effectiveness.

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Fernanda Alves
São Carlos Institute of Physics - University of São Paulo
Brazil

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