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Laser induced fluorescence (LIF) spectroscopic investigation of cervix tissue for the detection of chronic cervicitis

Ajaya Kumar Barik1, Sanoop Pavithran M1, Jijo lukose1, Rekha Upadhya2, Muralidhar V Pai2, Ajeetkumar Patil1, Aseefhali Bankapur1 and Santhosh Chidangil1

1. Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal
2. Department of Obstetrics & Gynaecology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal.


Cervical cancer is one of the leading cause of cancer mortalities. The detection of any abnormality like chronic cervicitis (CC), low-grade intraepithelial lesion (LGSIL), and high-grade intraepithelial lesion (HGSIL) may prevent further progression of diseases. Optical techniques are explored for the early detection and diagnosis of diseases in which laser-induced fluorescence (LIF) is one of the simple methods for the detection of various types of cancers. This technique is capable of discriminating normal and abnormal tissue samples without the necessity of exogenous fluorophores. In the present work, LIF studies have been performed using cervix tissues obtained from volunteers who have undergone hysterectomy. The tissue fluorescence was acquired by exciting the sample with a 325 nm He-Cd laser through a fiber optic probe. The fluorescence spectra show two distinct peaks due to collagen and NADH around 390 nm and 440nm respectively. The fluorescence intensity of collagen and NADH is different for normal and chronic cervicitis tissue. As the disease progresses, the NADH intensity increases, and the collagen intensity decreases. The device has proven to be a reliable technique for early detection, in vivo screening, and surgical demarcation.

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Ajaya Kumar Barik
Department of Atomic and Molecular Physics,MAHE,Manipal


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