Laser Induced Fluorescence study of kidney tissues: Diagnosis of renal cell carcinoma
Sanoop Pavithran M1, Arun Chawla2, Ajaya kumar Barik1, Vittal Shenoy1, Jijo Lukose1, Ammasi Periasami3, V.B. Kartha1, Santhosh Chidangil1
1. Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal
2. Department of Urology, Kasturba Medical College Manipal, Manipal Academy of Higher Education, Manipal
3. W.M. Keck Center for Cellular Imaging (KCCI), Biology, University of Virginia, USA
Abstract
Autofluorescence can reflect the metabolic state of biological systems. This pilot study investigates the worth of tissue fluorescence in the differentiation of two types of kidney cancer to that of normal. The 325 nm excitation-induced fluorescence from coenzymes NADH and FAD provided the signature of oxidative phosphorylation (OXPHOS) and aerobic glycolysis which in turn is a differentiating parameter for clear cell renal cell carcinoma(ccRCC) and chromophobe renal cell carcinoma(chRCC). The dependence of ccRCC tissue on aerobic glycolysis for their energy needs compared to normal counterparts was visible as increased intensity of free NADH (Emission at 465 nm). In the case of chRCC, the diminished fluorescence emission from bound NADH compared to normal renal tissue confirmed their maximized reliance on OXPHOS (Emission at 440 nm). This pilot study opens the possibility of a fluorescence-guided endoscopy-based probe for the differentiation of various kidney cancers.
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sanoop pavithran m
Department of Atomic and Molecular Physics,MAHE,Manipal
India
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