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Micro-Raman spectroscopy study of Iron Deficiency Anemia and Beta thalassemia

Mithun N,1, Jijo Lukose,1, Javed Ahammad M M,3, Shamee Shastry,2, Ganesh Mohan,2, Annamma Kurien,3, Santhosh Chidangil,1
1 Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics,
Manipal Academy of Higher Education, Manipal, Karnataka, India -576104
2 Department of Immunohematology and Blood Transfusion, Kasturba Medical College,
Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India -576104
3 Department of Pathology, Melaka Manipal Medical College, Manipal Academy of Higher
Education, Manipal, Karnataka, India -576104

Abstract

The most frequent microcytic anemia is known to be iron deficiency anemia (IDA) and Beta thalassemia trait (β-TT), which continue to be a considerable burden on society, particularly in low-income countries. The anemic patients have lack healthy RBCs or the reduction in the hemoglobin level, which will result in reduced transportation of oxygen to the body tissues (hypoxia). This work demonstrates the potential of the Raman Tweezers technique as a single cell spectroscopic tool for discriminating the RBCs of IDA and β-TT patients from healthy individuals. The single-cell spectroscopy of live cells suspended in a liquid media is difficult due to the Brownian motion of the cells. The combination of optical tweezers with Raman spectroscopy offers a better approach for extracting biochemical information at the individual cell levels. The Raman spectra of RBCs collected from IDA patients showed lower hemoglobin band intensities comparing to the healthy and beta-thalassemia traits. The principal component analysis (PCA) shows clear discrimination of the Raman spectra of IDA and beta-thalassemia trait from healthy RBCs.

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Mithun N
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, Karnataka, India -576104
India

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