BIOPHOTONICS OF BODY FLUIDS FOR CLINICAL APPLICATIONS
Investigation of body fluids is of paramount importance since it carries a pool of biological markers, which can reflect the health status of humans. Technological advancements in the field of biophotonics offer highly sensitive, objective and rapid detection of abnormal health conditions from body fluid analysis. Raman spectroscopy has been widely regarded as reliable spectroscopic tool for the characterization of body fluids due to its minimal interference imposed by aqueous environment. Raman Investigation of blood cells under physiological condition is restricted due to Brownian motion of micron sized cells. In view of this, spectroscopic characterizations of live cells under various stress agents and health abnormalities has been performed using a custom built Raman Tweezers, which involves the optical arresting of cells with the aid of tightly focused laser beam. Besides Raman Tweezers spectroscopy, High-performance liquid chromatography-Laser Induced Fluorescence (HPLC-LIF) technique have been also explored for the protein profiling of body fluids such as blood serum, saliva and tears collected from healthy and abnormal subjects. The ultra-sensitive HPLC–LIF system developed in our lab have been effective in carrying out screening, early detection, and staging for various cancers. Research and development is progressing well for the analysis exhaled breath for the detection of volatile organic compound as markers for various diseases including COVID-19 infection. An overview of these research activities will be discussed during the workshop.
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Centre of Excellence for Biophotonics Department of Atomic and Molecular Physics, Manipal Academy of Higher Education Manipal, Karnataka, India-576104