Polarized fluorescence parameters of FAD in solutions under excitation within the first and the second absorption bands
Marina K. Krasnopevtceva, Maxim E. Sasin, Ioanna A. Gorbunova, Dmitrii P. Golyshev, Andrey G. Smolin, Victor P. Belik, and Oleg S. Vasyutinskii
Ioffe Institute
Abstract
Flavin adenine dinucleotide (FAD) in its oxidized (FAD+) and reduced (FADH2) forms is an intracellular coenzyme involved in redox reactions in living cells. As known, FAD+ is able to fluoresce, while FADH2 is not. This important feature allows FAD to be used as a fluorescent probe for studying biochemical processes in living cells. In this article, anisotropy, lifetimes and rotational diffusion time of FAD in water-methanol solutions were determined experimentally and analyzed. Fluorescence was excited by picosecond laser pulses at 355 nm and 450 nm. Based on the analysis of experimental results obtained and the ab initio calculations performed, theoretical models that describe relaxation processes in FAD after excitation by a short laser pulse were suggested.
Speaker
Marina K. Krasnopevtceva
Ioffe Institute
Russia
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