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Mid-IR laser disinfection of pathogenic bacteria

Svetlana N. Shelygina,1, Victor O. Kompanets,2, Eteri R. Tolordava,3, Sergey I. Kudryashov,1 1 Lebedev Physical Institute RAS, Moscow, Russia 2 Institute of Spectroscopy RAS, Troitsk, Russia 3 Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia

Abstract

In this work the possibility of using of the mid-IR femtosecond laser irradiation to inactivation of pathogenic microorganisms was studied. Micrometer-thick layers of Pseudomonas aeruginosa were prepared on fluorite substrates and treated by mid-IR femtosecond laser irradiation that was tuned to achieve the selective excitation of either the stretching C-H vibrations (3 mkm), or stretching C=O, C-N vibrations (6 mkm) of amide groups of bacteria. It was shown that 3-mkm laser irradiation evenly heats the bacterial cell, while 6-mkm laser irradiation leads to selective heating and subsequent dissociative denaturation of lipids and proteins in the cell membrane and intra-cell nucleic acids. Viability assays have shown greater inactivation efficiency for 6-mkm laser treatment.

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Svetlana Shelygina
Lebedev Physical Institute RAS, Moscow, Russia
Russia

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