A reinforced collagen network of the Giant squid mantle - is it a candidate for a scaffold material in tissue engineering?

Abstract

Currently, a search and development of biocompatible materials with a high strength and elastic modulus is of importance, with the aim of their application as scaffolds in tissue engineering. We have studied a collagenous membrane (outer tunic) separated from the mantle of the Giant squid (Dosidicus Gigas species, family of Ommasrephinae). To establish the composition and structure of the studied material, we analyzed the membrane by a variety of techniques, including aminoacid analysis, SDS-PAGE, IR. It has been shown that collagen is a main component of the squid’s outer tunic. The morphology study by different microscopic techniques from the nano- to microscale revealed a peculiar collagen packing of interlaced bands. The macro- and micromechanical studies showed high values of the Young’s modulus and tensile strength. No significant cytotoxicity of the studied material was found by the cytotoxicity assay. Thus, the membrane from the Giant squid mantle consists of a reinforced collagen network, has high mechanical characteristic and is non-toxic that makes it a good candidate for the creation of a scaffold material for tissue engineering.

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Speaker

Anastasia Frolova
World-Class Research Center “Digital Biodesign and Personalized Healthcare”, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st, Moscow, 119991, Russian Federation
Russian Federation

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