super-resolution with very low laser power, ideal for long term imaging

Abstract

Since the introduction of the confocal microscope, 3D imaging has become the norm. By using the confocal.nl RCM upgrade box, a confocal microscope is created on the basis of an imaging microscopy setup, that has 3x better sensitivity, that has 40% better resolution than other (traditional) confocal microscopes on the market. Because of the disruptive product concept, we can offer our confocal solution at a very affordable price level. On top of that, the RCM confocal microscope is very easy to use, offering consistent results for all users.

One of the unique features of the RCM confocal microscope is that it is camera-based. Cameras are made with different resolutions, different sensitivities but also with different wavelength sensitivities. Recently new cameras have been released offering 95% QE. These new cameras make the RCM confocal microscope 3x more sensitive than a typical GaAsP PMT (30% QE) based confocal microscope.

By optimizing the internal optics for NIR wavelengths (650 - 850nm) and changing the camera type to a NIR camera, a sensitive NIR confocal microscope is created. Normally the use of NIR wavelengths is associated with the lower resolution because of the longer wavelengths, but the re-scanning principle of the RCM improves the resolution again. At 780nm excitation the resolution of a confocal microscope is 330nm but RCM improves this to 250nm!

RCM-VIS and RCM-NIR are available as complete microscope systems but also as upgrade versions from confocal.nl.

Speaker

Peter Drent
confocal.nl
Netherlands

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