DYNAMIC IMAGING OF THE LYMPHATIC DRAINAGE FROM THE EYE IN MICE BY MULTISPECTRAL PHOTOACOUSTIC TOMOGRAPHY
Vladislav Toronov, Ryerson University, 350 Victoria St, Toronto, ON, M5B 2K3, Canada and The Institute for Biomedical Engineering, Science & Technology (iBEST) St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada.
Balal Mian, Ryerson University, 350 Victoria St, Toronto, ON, M5B 2K3, Canada
Yeni Yucel, Ryerson University, 350 Victoria St, Toronto, ON, M5B 2K3, Canada and The Institute for Biomedical Engineering, Science & Technology (iBEST) St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada, and Keenan Research Centre for Biomedical Science of St. Michael's Hospital, 30 Bond St, Toronto, ON, M5B 1W8, Canada.
Xun Zhou, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, 30 Bond St, Toronto, ON, M5B 1W8, Canada.
Abstract
Recently lymphatic drainage from the eye has been potentially linked to glaucoma and other optic nerve diseases. However, this hypothesis requires further dynamic non-invasive imaging studies of the lymphatic drainage from the eye . Our work is focused on the development of the dynamic imaging of the lymphatic drainage in mice by multispectral photoacousing tomography (MSOT). The main challenge is the minimization of the number of wavelengths, which is required both to perform the dynamic imaging with sufficient temporal resolution and to provide robust separation of the chromophores such as the exogenous near-infrared lymph tracer, oxy- and deoxy- hemoglobins, and melanin. We present a wavelength optimization technique which allows for the accurate dynamic imaging of the lymphatic drainage from the eye with the temporal resolution of few seconds per image, and details on the lymphatic drainage process.
Speaker
Vladislav Toronov
Ryerson University
Canada
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