In vivo multimodal optical imaging of equivocal melanocytic skin lesions
V. Elagin 1, E. Gubarkova 1, O. Garanina 1, D. Davydova 1, N. Orlinskaya 1, L. Matveev 2, I. Klemenova 1, I. Shlivko 1, E. Zagaynova 1
1 – Privolzhsky Research Medical University, Nizhny Novgorod, Russia
2 – Institute of Applied Physics of the Russian Academy of Sciences, Nizhny Novgorod, Russia
Abstract
There is a wide range of equivocal melanocytic lesions that can be clinically and dermoscopically indistinguishable from early melanoma. To carry out the first evaluation of the possibilities of using integrated study of dermoscopic equivocal melanocytic lesions involving, multiphoton microscopy (MPM) and optical coherence angiography (OCA). Fifty-nine melanocytic lesions were studied using dermoscopy, MPM and OCA. A system of multiphoton optical scoring (MPOS) has also been developed for quantitative assessment of the melanoma features revealed by MPM. To reveal difference in vessel networks, OCA images were processed to calculate the vessel densities and the total lengths thin and thick vessels. Clinical and dermoscopic examinations revealed 9 benign lesions and 32 melanomas. 18 lesions remained difficult to diagnose. Histopathological analysis separated these equivocal lesions into three types: benign (lentigo simplex, dysplastic nevi), melanoma in situ, and invasive melanoma. The mean value of the MPOS was shown to be lower for benign lesions than for the malignant ones. Quantitative analysis of OCA images revealed that the invasive melanoma type has the highest vessel density due to an increase in the number of thick (greater than 50 µm in diameter) vessels. We analyzed the data with multivariate statistical method - discriminant function analysis. The results indicate that the MPOS and OCA can correctly distinguish all equivocal melanocytic lesions.
Speaker
Vadim Elagin
Privolzhsky Research Medical University
Russia
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