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Metabolism of stem cell grown on heterogeneous tissue-engineered scaffolds

V. Elagin 1, S. Rodimova 1,2, N.V. Minaev 3, A.I. Shpichka 4, E.V. Zagaynova 1,2, P.S. Timashev 3,4, D.S. Kuznetsova 1
1 – Privolzhsky Research Medical University, Nizhny Novgorod, Russia
2 – Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
3 – Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics” Russian Academy of Sciences, Moscow, Russia
4 – Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia


One of the promising approaches to the treatment of injured tissue is the application of bioengineering techniques based on the introduction of cells into the damage area. An application of cells alone does not provide a complete replacement of the tissue defect. Therefore, scaffolds are used allowing organization of the cells into a structure, which is capable of the total reproduction of the damaged tissue integrity. Great number of factors, which influence the cell behavior and tissue formation at the injury site when using scaffolds are known. Here, we analyzed the effect of structural heterogeneity of scaffolds on cellular behavior and metabolism. All scaffolds were obtained by two-photon polymerization technique. It was found that colonization of heterogeneous scaffolds was insignificant less than homogeneous ones. However, there were not dead cells on heterogeneous matrix. We found that the level of unbound and protein-bound NAD(P)H for the cells on the heterogeneous and standard scaffolds was not the same. This may indicate a different contribution of glycolysis and oxidative phosphorylation to the overall cell metabolism.

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Vadim Elagin
Privolzhsky Research Medical University


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