Synthesis of molecularly imprinted polymers for enzymes
Molecularly imprinted polymers (MIPs) are synthetic analogs of biological receptor systems obtained by imprinting template molecules in a polymer layer. The polymeric matrix forms specific recognition sites after removing template by destruction and intensive washing or by suitable desorption agents. MIPs are capable to bind a specific analyte from a complex matrix with high selectivity since they can act as receptors for the target molecule recognition.
Compared to natural receptors, such as active proteins, enzymes, antibodies, etc, MIPs possess unique properties, enclosing selectivity recognition, high chemical stability and low synthesis cost The successful imprinting of macromolecules, including proteins, DNA, viruses remains a difficult task due to the large size of the molecules, the large number of functional groups, low conformational flexibility and sensitivity to external parameters.
In this work, we have synthesized MIPs for various enzymes: horseradish peroxidase, glucose oxidase and soybean peroxidase. Polyaniline chemically synthesized was used as MIPs polymer matrix and were used microstructured optical fibers as a base plate for synthesis. We investigated the analytical properties and stability of the synthesized MIPs.
The reported study was funded by Russian Foundation for Basic Research according to the research project № 18-29-08033.
Presnyakov Kirill Yurievich
Saratov State University, Institute of Chemistry
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