In vitro study of exosome secretion rate by nanoparticle tracking analysis
A.Merdalimova, V.Chernyshev, R. Chuprov-Netochin, D.Gorin
Abstract
Exosomes are nano-sized vesicles secreted by almost all cell types. Exosomes are structures with a lipid bilayer, containing proteins and miRNA from their parental cells and actively participating in cell-cell communication, that makes them perspective for diagnostic and treatment purposes. However, exosome isolation and analysis are difficult due to their small size and complicated structure. Nanoparticle tracking analysis (NTA) allows to measure size and concentration of exosomes. In particular, dynamics of exosome secretion is a task of interest. In last decade, several works reported that cancer cells release exosomes more intensively compared to normal ones. Furthermore, in vitro experiments demonstrated that exosome secretion rate depends on cell type, environmental factors and inter alia presence of exosomes around: exosomes surrounding the cell inhibit further secretion. In the present work dynamics of exosome secretion on cell cultures of different densities is considered and compared with literature. This data may be considered in further development of sensing systems for exosome isolation and detection that can be used for early diagnosis of cancer diseases.
Speaker
Anastasiia Merdalimova
Skolkovo Institute of Science and Technology
Russia
Discussion
Ask question
