Getting things back from the bloodflow: flow cytometer and magnetic sorter foe detection and extraction of objects from undiluted blood.
D.N. Bratashov (Saratov state university), R.A. Verkhovskii (Saratov state university), O.A. Sindeeva (Skoltech, Saratov state university), A.A. Kozlova (Saratov state university), I.O. Kozhevnikov (Saratov state university), E.S. Prikhozhdenko (Saratov state university), O.A. Mayorova (Saratov state university), O.V. Grishin (Saratov state university), M.A. Makarkin (Saratov state university), A.V. Ermakov (Saratov state university), A.S. Abdurashitov (Skoltech)
Abstract
We have developed SPIM-based flow cytometer combined with the magnetic object sorter to extract objects from the undiluted blood. The optical arrangement of the device is similar to SPIM-Fluid lightsheet-based imaging flow cytometer, and magnetic sorter using the permanent rare-earth magnet and the magnetic field concentrator. The device allows to see the whole cross-section of fluidic system without hydrodynamic focusing and thus without blood dilution that allows to connect it directly into the bloodflow of the laboratory animal shunting two large blood vessels. The device allows to see and count fluorescently-labeled objects in undiluted blood both in vivo and in vitro. It can be applied to detect circulating tumor cells and other pathogens in the bloodflow, that are extremely rare or to count amount of targeted drug delivery carriers that reach the specific vessel after of its injection into the bloodflow. We have also retrieved some of the carriers functionalized with the magnetic nanoparticles directly from the bloodflow and investigated it with different microscopy modalities in vitro.
This research was supported by the RUSSIAN SCIENCE FOUNDATION, grant number 18-19-00354.
Speaker
Daniil Bratashov
Saratov state university
Russia
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