Bimodal fluorescence and optoacoustic contrast agent based on the self-quenching of indocyanine green
Maksim D. Mokrousov, Skolkovo Institute of Science and Technology
Marina V. Novoselova, Skolkovo Institute of Science and Technology
Jackie Nolan, University of Arkansas for Medical Sciences
Walter Harrington, University of Arkansas for Medical Sciences
Polina Rudakovskaya, Skolkovo Institute of Science and Technology
Daniil N. Bratashov, Saratov State University
Ekaterina I. Galanzha, University of Arkansas for Medical Sciences, Saratov State University
Juan Pablo Fuenzalida-Werner, Institute of Biological and Medical Imaging (IBMI)
Boris P. Yakimov, Lomonosov Moscow State University
Gleb Nazarikov, Skolkovo Institute of Science and Technology
Vladimir P. Drachev, Skolkovo Institute of Science and Technology, University of North Texas
Evgeny A. Shirshin, Lomonosov Moscow State University, Institute of Spectroscopy of the Russian Academy of Sciences
Vasilis Ntziachristos, Institute of Biological and Medical Imaging (IBMI), Chair of Biological Imaging and Center for Translational Cancer Research (TranslaTUM)
Andre C. Stiel, Institute of Biological and Medical Imaging (IBMI)
Vladimir P. Zharov, University of Arkansas for Medical Sciences, Saratov State University
Dmitry A. Gorin, Skolkovo Institute of Science and Technology
Abstract
A bimodal contrast agent was obtained using the self-quenching of indocyanine green (ICG) encapsulated in a biodegradable polymer shell. Freezing-induced loading and layer-by-layer assembly allowed to increase local ICG concentration that is necessary for the obtaining of self-quenching effect. As a result, an efficient optoacoustic/fluorescent contrast agent based on composite indocyanine green/polymer particles was successfully prepared and was characterized by fluorescence and optoacoustic(photoacoustic) tomography in vitro. Due to its high efficiency and biosafety, this type of contrast agent has a good perspective for clinical application.
1. Maksim D. Mokrousov, Marina V. Novoselova, Jackie Nolan, Walter Harrington, Polina Rudakovskaya, Daniil N. Bratashov, Ekaterina I. Galanzha, Juan Pablo Fuenzalida-Werner, Boris P. Yakimov, Gleb Nazarikov, Vladimir P. Drachev, Evgeny A. Shirshin, Vasilis Ntziachristos, Andre C. Stiel, Vladimir P. Zharov, and Dmitry A. Gorin, "Amplification of photoacoustic effect in bimodal polymer particles by self-quenching of indocyanine green," Biomed. Opt. Express 10, 4775-4788 (2019)
Speaker
Maksim Mokrousov
Skolkovo Institute of Science and Technology
Russia
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