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Response of malignant tumor cells to photodynamic treatment assessed by means of digital holographic microscopy

A. A. Zhikhoreva1, A. V. Belashov1, N. A. Avdonkina2, I. A. Baldueva2, A. B. Danilova2, M. L. Gelfond2, T. L. Nekhaeva2, I. V. Semenova1, and O. S. Vasyutinskii1

1Ioffe Institute, Russian Academy of Sciences, St. Petersburg, Russia
2N.N. Petrov National Medical Research Center, Ministry of Health of Russia, St. Petersburg, Russia


In this work, we present an investigation of cell death dynamics and changes of their optical and morphological parameters resulted from photodynamic treatment. The cell cultures were obtained from tumor tissues of individual patients. The studied malignancies were kidney carcinoma, osteosarcoma, and melanoma. In-vitro monitoring of living cells was performed using digital holographic microscope in Mach–Zehnder layout.
The dynamics of average phase shift and dry mass after photodynamic treatment were obtained for each cell line. It was shown that at the same treatment dose cell cultures of some patients did not respond to photodynamic treatment, while others demonstrated an exponential decrease of dry mass and average phase shift, that is indicative of the necrotic process. Necrosis rates were evaluated by analysis of the obtained exponential temporal behaviors of both parameters and were shown to be informative for analysis of tumor resistance to PDT. The results obtained can be used for evaluation of patient’s response to PDT and for the optimization of treatment doses for individual patients.


Anna Zhikhoreva
Ioffe Institute, Russian Academy of Sciences, St. Petersburg, Russia


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