Confocal Raman Microspectroscopy of porcine skin ex vivo using laser excitation at 633 nm and optical clearing with glycerol/water/DMSO solution
Confocal Raman spectroscopy (CRM) is a powerful noninvasive biochemical investigation method allowing label-free identification of dynamic characterization and imaging of living biological tissues on cellular level. It can be used to measure depth-dependent profiles of porcine skin ex vivo as well. This work attempts to show the effect of immersion optical clearing on porcine skin to increase the sensing depth, spatial resolution, and contrast of the resulting images/spectra by using CRM with excitation light at 633 nm at different depths. The optical clearing has been provided by applying mixture of glycerol with distilled water and dimethyl sulfoxide after 30 and 60 min of treatment. The obtained results demonstrate that increasing the exposure time allows to improve the optical clearing of the skin making the deep skin layer of dermal regions able for investigation and distinguishing structural differences between the type I and IV collagens. The differences between epidermis, dermis, and epidermis/dermis interface can also be revealed in the amide III spectral region.
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a- Institute for Solid State Physics and Optics, Wigner Research Center for Physics, P.O. Box 49, Budapest, H-1525, Hungary .b- Institute of Physics, University of Szeged, Dom ter 9, H-6720 Szeged, Hungary
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