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Cyclic tissue clearing

Nataliya Kozintseva, MEPHI, Moscow, Russia
Arkady S. Abdurashitov, Saratov State University, Saratov, Russia; Tomsk State University, Tomsk, Russia
Valery V. Tuchin, Saratov State University, Saratov, Russia; Tomsk State University, Tomsk, Russia; Institute of Precision Mechanics and Control RAS, Saratov, Russia
ITMO University, St. Petersburg, Russia


Nowadays, methods of non-invasive analysis are in great demand. Tissue clearing is one of the main methods of non-invasive diagnostics of diseases. In this work, tissue clearing is combined with laser speckle imaging, which is a powerful, accurate and, at the same time, relatively inexpensive and easy-in-use instrument for non-invasive research. In the experiment, glycerin was used as a clearing agent and the dura mater was used as an object. The object is cleared according to a cyclical algorithm: until the object is significantly cleared in glycerin, then it is placed in a NaCl solution for a couple of minutes until its scattering properties are restored, then the object is again placed in an optical clearing agent. Such a multi-stage scheme leads to a higher degree of clearing of the dura mater, as shown in the obtaine results of the experiment.


Nataliya Kozintseva
MEPHI, Moscow, Russia


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