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THE EFFICIENCY OF THE REFRACTIVE INDEX MATCHING MECHANISM IN VARIOUS BIOLOGICAL TISSUES – A COMPARATIVE STUDY BETWEEN THE ULTRAVIOLET AND THE VISIBLE-NIR REGIONS

Nelma M. Gomes, Physics Department, Polytechnic of Porto – School of Engineering, Porto 4249-015, Portugal

Luís M. Oliveira, CIETI/Physics Department, Polytechnic of Porto – School of Engineering, Porto 4249-015, Portugal

Valery V. Tuchin, Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov 410012; Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Tomsk 634050; Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov 410028; and Laboratory of Molecular Imaging, А.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia

Abstract

In this study, we present the kinetics and efficiency of the refractive index (RI) matching mechanism created by different osmolarities of glycerol in human normal and pathological tissues. Considering a wavelength range between 200 and 1000 nm, it has been observed that the RI matching is more efficient in the ultraviolet (UV) range for colorectal muscle and colorectal pathological mucosa tissues, while for normal mucosa, where the protein content is smaller, similar efficiency has been observed in the UV and visible-NIR range. By treating muscle with different glycerol osmolarities, it has been observed that the UV efficiency for the RI matching mechanism increases with increasing glycerol osmolarity in the treating solution. Such fact indicates that the dissociation of proteins in the tissue has a major role in the efficiency of the RI matching in the UV range.

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Luís Oliveira
CIETI/Physics Department, Polytechnic Institute of Porto - School of Engineering
Portugal

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