Application of Digital Speckle Patterns Correlation for Blood Clotting Time Evaluation
Today, the use of optical diagnostic methods in medicine is widely spread due to the non-contact method and the safety of low-intensity radiation for the body. Information about the time of blood coagulation during the operation will help the doctor to immediately take steps to eliminate the problem, which will help to avoid situations dangerous for the patient’s life. To date, there are no devices for continuous monitoring of blood clotting time. The purpose of the research work is to develop a prototype device for the rapid assessment of blood clotting time.
According to statistics, the most serious consequences after surgery are infectious infections and thrombosis. The device under development is designed for continuous monitoring of blood clotting time. If the doctor is able to maintain blood viscosity in a normal state during operations, then the risk of thrombosis after surgery will be significantly less. Therefore, the development of this device is relevant today.
Existing coagulometers are exclusively laboratory equipment and analyze the clotting time of blood plasma. It is not rational to use them in operating rooms. In order to analyze the blood coagulation time in existing coagulometers, the laboratory technician needs to separate the blood plasma from the formed elements in a centrifuge and place the resulting plasma in a cuvette. Then add non-magnetic balls to the cuvette and then take measurements. Also, existing coagulometers have a drawback: subsidence of elements. Through 300 cycles of operation the device requires calibration and replacement of optical elements, which is a big drawback of the device. It is also necessary to note the existing thromboelastographs, which carry out a complete analysis of whole blood, however, require constant purification of the electrodes.
The sample volume for these devices is 1.5 ml, which is 300 times the volume of the sample analyzed by the proposed method.
Thus, it can be said that the device under development has 5 main advantages: minimum sample volume, whole blood analysis, lack of consumables, the possibility of continuous monitoring and non-contact method.
To date, the laboratory setup has been assembled, the application of the method for analyzing the clotting time of blood plasma has been confirmed and the possibility of applying the method for analyzing whole blood has been shown. In further studies, it is necessary to create a prototype of the device being developed, to experimentally confirm the applicability of the proposed method for analyzing the clotting time of whole blood. It is also necessary to develop software for the microcontroller.
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Iuliia D. Liushnevskaya
Tomsk Polytechnic University
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