Determination of the water profile in the stratum corneum of the oil-treated skin using in vivo confocal Raman microscopy
In this study, a new calculation method for the determination of the water profiles in oil-treated skin is proposed, which is based on the normalization of the Raman band intensity of water (3350-3550 cm−1) by Amide I at 1650 cm−1. The developed method is compared with the conventional method, which is based on the normalization of the Raman band intensity of water (3350-3550 cm−1) by keratin at 2930 cm−1.
Due to the superposition of oil- and keratin-related Raman bands at 2930 cm−1, the conventional method creates artifacts by calculating water concentration profile in oil-treated skin. A lower amount of water in the upper and intermediate regions of the stratum corneum are measured. The new method shows no artifacts and correctly determines the water depth profiles after topical application of cosmetic formulations on the skin.
File with report